CD28 抗体 (PE)
(6 references)-
- 抗原 See all CD28 抗体
- CD28
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适用
- 人, Non-Human Primate
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD28 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
- 交叉反应 （详细）
- Human, Non-Human Primates
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
- 克隆位点
- CD28-2
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD28 Primary Antibody
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- 限制
- 仅限研究用
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- 溶解方式
- No reconstitution is necessary.
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, (2004) (PubMed).
: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, (2004) (PubMed).
: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, (2001) (PubMed).
: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, (1999) (PubMed).
: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, (1999) (PubMed).
: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, (1993) (PubMed).
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: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, (2004) (PubMed).
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- 抗原
- CD28
- 别名
- CD28 (CD28 产品)
- 别名
- Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
- 背景
- CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,TP44
- 基因ID
- 940
- UniProt
- P10747
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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