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CD28 抗体 (APC)

Name: CD28 抗体 (APC)
Brand: antibodies-online
SKU: ABIN349705
Availability: OutOfStock

CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 APC

Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28. (CD28 抗体 (APC))

FACS

2 images

(6 references)

产品编号 ABIN349705

发货至: 中国

Datasheet as PDF

抗原 See all CD28 抗体

CD28
适用
168

119

55

34

22

16

12

12

7

6

5

5

1

1

1

1
人, Non-Human Primate
宿主
126

77

28

16

8

3

2
小鼠
Compatible Secondaries
克隆类型
182

72

3
单克隆
标记
89

31

28

21

13

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This CD28 antibody is conjugated to APC
应用范围
197

90

53

47

38

32

28

26

19

17

13

7

6

6

4

4

4

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA

克隆位点

CD28-2

亚型

IgG1 kappa

Top Product

Discover our top product CD28 Primary Antibody
anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC

anti-CD28 (CD28) antibody
CD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) (AA 19-152) antibody
CD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated

anti-CD28 (CD28) (C-Term) antibody
CD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD28 (CD28) (Internal Region) antibody
Verified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Jeong, Qiao, Nascimbeni, Hu, Rehermann, Murthy, Liang: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, (2004) (PubMed).

Scharschmidt, Wegener, Heissmeyer, Rao, Krappmann: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, (2004) (PubMed).

Marti, Krause, Post, Lyddane, Dupont, Sadelain, King: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, (2001) (PubMed).

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, (1999) (PubMed).

Tazi, Moreau, Bergeron, Dominique, Hance, Soler: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, (1999) (PubMed).

Nunes, Klasen, Franco, Lipcey, Mawas, Bagnasco, Olive: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, (1993) (PubMed).
抗原

CD28

别名

CD28 (CD28 产品)

别名

Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

背景

CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,TP44

基因ID

940

UniProt

P10747

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response