EpCAM 抗体
(6 references)-
- 抗原 See all EpCAM (EPCAM) 抗体
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EpCAM antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 特异性
- The mouse monoclonal antibody 323/A3 recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.
- 无交叉反应
- 大鼠
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Human breast cancer MCF-7 cells
- 克隆位点
- 323-A3
- 亚型
- IgG1
- Top Product
- Discover our top product EPCAM Primary Antibody
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibodyEPCAM 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibodyEPCAM 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rEGP40-1372 unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 FACS, IF, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1373 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1372 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibodyEPCAM 适用: 人 FACS, IHC, StM 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibodyEPCAM 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibodyEPCAM 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7B5C10 unconjugated
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- 应用备注
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Western blotting: Non-reducing conditions, recommended dilution: 1-2 μg/mL, positive control: MCF-7 cells.
Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 mL lysate.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/mL, pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37 °C, 1 mg / ml Tris-HCl, pH 2.0), positive tissue: breast carcinoma.
Immunocytochemistry: Recommended dilution: 1-10 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM)." in: Digestive diseases and sciences, Vol. 48, Issue 12, pp. 2306-9, (2004) (PubMed).
: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, (2003) (PubMed).
: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, (2001) (PubMed).
: "Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4833-43, (1998) (PubMed).
: "Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion." in: Cell adhesion and communication, Vol. 2, Issue 5, pp. 417-28, (1995) (PubMed).
: "Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule." in: The Journal of cell biology, Vol. 125, Issue 2, pp. 437-46, (1994) (PubMed).
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: "Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM)." in: Digestive diseases and sciences, Vol. 48, Issue 12, pp. 2306-9, (2004) (PubMed).
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- 抗原
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 别名
- CD326 / EpCAM (EPCAM 产品)
- 别名
- DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody
- 背景
- Epithelial cell adhesion molecule,CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.,EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1
- 基因ID
- 4072
- UniProt
- P16422
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