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AKT1 抗体 (pSer473)

Name: AKT1 抗体 (pSer473)
Brand: antibodies-online
SKU: ABIN349614
Availability: OutOfStock

AKT1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, FACS, IP, FM, MA 宿主: 小鼠 Monoclonal 17F6-B11 unconjugated

Western Blot of Mouse Anti-Akt pS473 antibody. (AKT1 抗体 (pSer473))

Immunohistochemistry of Mouse anti-AKT pS473 antibody. (AKT1 抗体 (pSer473))

IHC

4 images

(3 references)

产品编号 ABIN349614

发货至: 中国

Datasheet as PDF

抗原 See all AKT1 抗体

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
抗原表位
79

43

34

31

29

22

19

18

12

12

10

9

8

8

8

8

8

7

6

6

6

5

5

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
pSer473
适用
472

300

240

54

46

45

33

16

12

11

5

3

3

2

2

1

1

1
人, 小鼠, 大鼠, 猴
宿主
403

84

7

2

1
小鼠
Compatible Secondaries
克隆类型
367

129
单克隆
标记
282

30

22

20

15

14

8

8

8

8

8

8

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1
This AKT1 antibody is un-conjugated
应用范围
390

222

157

76

69

65

50

41

31

30

27

15

13

9

8

6

4

3

3

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

原理

AKT phospho S473 Antibody

交叉反应（详细）

This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473.

产品特性

Synonyms: mouse anti-AKT pS473 Antibody, phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

纯化方法

Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

过滤

Sterile filtered

免疫原

Immunogen: Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
Immunogen Type: Conjugated Peptide

克隆位点

17F6-B11

亚型

IgG1 kappa

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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibody
AKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
AKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
AKT1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC, IF, FACS, FM 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1F7E10 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1F7G2 unconjugated

应用备注

Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 20 μg/mL
Application Note: Phospho AKT antibody is tested in ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Western Blot Dilution: 1:500-1:3,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:20,000
IF Microscopy Dilution: 1:500-1:3,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.02 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Anti-AKT pS473 (MOUSE) Monoclonal Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Maerz, Burkhalter, Schilpp, Wittekindt, Frick, Philipp: "Pharmacological cholesterol depletion disturbs ciliogenesis and ciliary function in developing zebrafish." in: Communications biology, Vol. 2, pp. 31, (2019) (PubMed).

Sha, Thompson, South, Baron, Leask: "Loss of PPARγ expression by fibroblasts enhances dermal wound closure." in: Fibrogenesis & tissue repair, Vol. 5, pp. 5, (2012) (PubMed).

Ito, Nishibori, Ito, Takagi, Akimoto, Kudo, Asanuma, Sai, Miyamoto, Takenaka, Yan: "mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndrome." in: Laboratory investigation; a journal of technical methods and pathology, (2011) (PubMed).
抗原

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

别名

AKT1 (AKT1 产品)

背景

Background: AKT phospho S473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

基因ID

207, 62241011

UniProt

P31749

途径

PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect