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GFP 抗体 (AA 1-246)

Name: GFP 抗体 (AA 1-246)
Brand: antibodies-online
SKU: ABIN349609
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 小鸡 Polyclonal unconjugated

Western Blot of anti-GFP antibody. (GFP 抗体 (AA 1-246))

Immunofluorescence Microscopy of chicken anti-GFP antibody. (GFP 抗体 (AA 1-246))

Western Blot of Anti-GFP (CHICKEN) antibody. (GFP 抗体 (AA 1-246))

3 images

(9 references)

产品编号 ABIN349609

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
16

15

10

8

4

4

3

3

2

2

1

1

1

1

1

1

1

1
AA 1-246
适用
175

15

11

9
Aequorea victoria
宿主
64

62

35

14

9

5

1

1
小鸡
克隆类型
114

72
多克隆
标记
81

13

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
149

75

46

33

25

25

25

20

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

特异性

Anti-GFP Antibody IgY was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

交叉反应

recombinant GFP (rGFP), enhanced GFP (eGFP)

产品特性

Anti-GFP is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. GFP Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic

过滤

Sterile filtered

免疫原

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: RecombinantProtein

亚型

IgG

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应用备注

Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated Polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots

限制

仅限研究用
状态

Liquid

浓度

0.64 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

有效期

3 months
Li, Cao, Xiao, Tang, Deng, Yang, Yoshii, Luo: "Hub-organized parallel circuits of central circadian pacemaker neurons for visual photoentrainment in Drosophila." in: Nature communications, Vol. 9, Issue 1, pp. 4247, (2019) (PubMed).

Goswami, Wang, Zhang, Xiao, Karlen, Li, Zawadzki, Burns, Lam, Pugh: "Novel window for cancer nanotheranostics: non-invasive ocular assessments of tumor growth and nanotherapeutic treatment efficacy in vivo." in: Biomedical optics express, Vol. 10, Issue 1, pp. 151-166, (2019) (PubMed).

Chiu, Martenson, Yamazaki, Natsume, Sakimura, Tomita, Tavalin, Higley: "Input-Specific NMDAR-Dependent Potentiation of Dendritic GABAergic Inhibition." in: Neuron, Vol. 97, Issue 2, pp. 368-377.e3, (2018) (PubMed).

Maruoka, Kedashiro, Ueda, Mizutani, Takai: "Nectin-4 co-stimulates the prolactin receptor by interacting with SOCS1 and inhibiting its activity on the JAK2-STAT5a signaling pathway." in: The Journal of biological chemistry, Vol. 292, Issue 17, pp. 6895-6909, (2017) (PubMed).

Boye, Alexander, Witherspoon, Boye, Peterson, Clark, Sandefer, Girkin, Hauswirth, Gamlin: "Highly Efficient Delivery of Adeno-Associated Viral Vectors to the Primate Retina." in: Human gene therapy, Vol. 27, Issue 8, pp. 580-97, (2017) (PubMed).

Kim, Fenk, Lyu, Maimon: "Quantitative Predictions Orchestrate Visual Signaling in Drosophila." in: Cell, Vol. 168, Issue 1-2, pp. 280-294.e12, (2017) (PubMed).

Chen, Luhur, Sokol: "Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine." in: Development (Cambridge, England), Vol. 142, Issue 20, pp. 3478-87, (2016) (PubMed).

He: "miRNA Tagging and Affinity-purification (miRAP)." in: Bio-protocol, Vol. 2, Issue 19, (2012) (PubMed).

Tseng, Firestein: "The role of PSD-95 and cypin in morphological changes in dendrites following sublethal NMDA exposure." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 43, pp. 15468-80, (2011) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

Anti-GFP is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. GFP Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower backgrounds for western blotting, ELISA and Immunohistochemistry.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.