NEDD4 抗体 (Internal Region)
-
- 抗原 See all NEDD4 抗体
- NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
-
抗原表位
- Internal Region
-
适用
- 人, 马, Macaque
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This NEDD4 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA
- 特异性
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology with the immunizing sequence. Expect reactivity with Nedd4 from chimpanzee, rabbit, dog, and cattle based on a 94% homology with the immunizing sequence. Cross-reactivity with Nedd4 from other sources has not been determined.
- 交叉反应
- 马, Macaque
- 产品特性
- NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-beta signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.
- 纯化方法
- affinity purified
- 过滤
- Sterile filtered
- 免疫原
-
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
Immunogen Type: Peptide - 亚型
- IgG
- Top Product
- Discover our top product NEDD4 Primary Antibody
-
-
- 应用备注
- This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kDa in size corresponding to Nedd4 by western blotting in the appropriate cell lysate or extract.
- 说明
-
Gene Name: NEDD4
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.64 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
- 有效期
- 3 months
-
- 抗原
- NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
- 别名
- NEDD4 (NEDD4 产品)
- 别名
- NEDD4-1 antibody, RPF1 antibody, AA959633 antibody, AL023035 antibody, AU019897 antibody, E430025J12Rik antibody, Nedd4-1 antibody, Nedd4a antibody, mKIAA0093 antibody, CG32184 antibody, CG42279 antibody, CG7555 antibody, DNedd4 antibody, Dmel\\CG42279 antibody, Dmel_CG32184 antibody, Dmel_CG7555 antibody, dNedd4 antibody, dNedd4-1 antibody, nedd4 antibody, GB16853 antibody, NEDD4 antibody, Nedd4 antibody, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody, neural precursor cell expressed, developmentally down-regulated 4 antibody, CG42279 gene product from transcript CG42279-RJ antibody, E3 ubiquitin-protein ligase Nedd-4 antibody, E3 ubiquitin-protein ligase NEDD4 antibody, NEDD4 antibody, Nedd4 antibody, LOC411723 antibody, LOC100457150 antibody, nedd4 antibody, LOC100547485 antibody
- 背景
-
NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.
Synonyms: E3 ubiquitin protein ligase Nedd4 antibody, KIAA0093 antibody, NEDD 4 antibody, Neural precursor cell expressed developmentally down regulated 4 antibody - 基因ID
- 4734, 114520609
- UniProt
- P46934
- 途径
- Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2
-