NEDD4 抗体
Quick Overview for NEDD4 抗体 (ABIN349606)
抗原
See all NEDD4 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-b05
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Supplier
- Rockland
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原理
- NEDD4 Antibody
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交叉反应 （详细）
- This antibody reacts with endogenous Nedd4 protein.
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产品特性
- Synonyms: rabbit anti-NEDD4 antibody, NEDD-4, NEDD 4, E3 ubiquitin protein ligase Nedd4 antibody, NEDD4-1 antibody, Neural precursor cell expressed developmentally down regulated 4 antibody, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, PIG53
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319) antibody
NEDD4 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 160-460) antibodyNEDD4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 801-900) antibodyNEDD4 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319), (C-Term) antibodyNEDD4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15462 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) antibodyNEDD4 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 950-1000) antibodyNEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 200-250) antibodyNEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 324-373) antibodyNEDD4 适用: 人, 马, 猴, 犬, 兔, Cow, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (Middle Region) antibodyNEDD4 适用: 人, 大鼠, 小鼠, 马, 犬, 兔, Cow, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kDa in size corresponding to Nedd4 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:300 to 1:500
ELISA Dilution: 1:20,000 - 1:35,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.64 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
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别名
- NEDD4
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背景
- Background: NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.
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基因ID
- 4734, 114520609
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UniProt
- P46934
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途径
- Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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