LacI 抗体
(4 references)
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- 抗原
- LacI
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适用
- E. coli, Shigella sonnei
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 600-401-b04
- Supplier
- Rockland
- 原理
- Lac I Antibody
- 交叉反应 （详细）
- This antibody reacts with recombinant Lac I protein.
- 产品特性
- Synonyms: rabbit anti-LacI antibody, rabbit anti-Lac-I antibody, Rabbit anti-Lac I antibody, Lactose operon repressor, DNA binding transcriptional repressor antibody, Transcriptional repressor of the lac operon antibody
- 纯化方法
- This Anti-Lac I product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
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anti-LacI antibody
适用: 人, 小鼠, 大鼠 WB, IC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Rabbit anti-Lac-I antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:90,000 - 1:100,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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-
: "Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis." in: Nature structural & molecular biology, Vol. 29, Issue 9, pp. 932-941, (2022) (PubMed).
: "Chromatin targeting of nuclear pore proteins induces chromatin decondensation." in: The Journal of cell biology, Vol. 218, Issue 9, pp. 2945-2961, (2020) (PubMed).
: "Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins." in: mBio, Vol. 8, Issue 3, (2018) (PubMed).
: "Developmental regulation of chromatin conformation by Hox proteins in Drosophila." in: Cell reports, Vol. 1, Issue 4, pp. 350-9, (2013) (PubMed).
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: "Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis." in: Nature structural & molecular biology, Vol. 29, Issue 9, pp. 932-941, (2022) (PubMed).
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- 抗原
- LacI
- 背景
- Background: Anti-Lac I antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase, modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.
- 基因ID
- 8156885, 146576
- UniProt
- P03023
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