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ybeY 抗体 (C-Term)

Rockland 600-401-a94 ybeY 适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN349601
Supplier Product No.: 600-401-a94
发货至: 中国
  • 抗原 See all ybeY 抗体
    ybeY (YbeY Metallopeptidase (Putative) (ybeY))
    抗原表位
    C-Term
    适用
    宿主
    • 5
    克隆类型
    • 5
    多克隆
    标记
    • 2
    • 1
    • 1
    • 1
    This ybeY antibody is un-conjugated
    应用范围
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    600-401-a94
    Supplier
    Rockland
    原理
    KSHV ORF57 Antibody
    交叉反应 (详细)
    This affinity purified antibody is directed against human herpesvirus 8 (KSHV ORF57) protein.
    产品特性
    Synonyms: rabbit anti-KSHV ORF57 antibody, MTA, human herpesvirus 8 protein
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product ybeY Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band 50-55 kDa in size corresponding to KSHV ORF57 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:5,000 - 1:8,000

    ELISA Dilution: 1:100,000 - 170,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.55 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    ybeY (YbeY Metallopeptidase (Putative) (ybeY))
    别名
    ORF57 (ybeY 产品)
    背景
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ORF57 (also known as MTA), one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed, is essential for virus lytic replication. A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein, and those examined act post-transcriptionally, affecting RNA splicing and transport.  KSHV ORF57 is capable of establishing both lytic and latent replication cycles.  In KS, the virus localizes to tumor progenitor endothelial cells, most of which are latently infected.  In cell culture, KSHV replication is generally studied using B-cell lines, such as BCBL-1, generated from primary effusion lymphoma material.  Most BCBL-1 cells are latently infected, although there is some spontaneous virus reactivation.  Addition of chemical inducers such as sodium n-butyrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), and valproic acid (VA) to these cells efficiently induces the lytic cycle and produces virions.  KSHV ORF57 protein predominantly localizes to the nucleus and can shuttle between the nucleus and cytoplasm.  Most HSV-1 genes are unspliced, by contrast, ORF57 is spliced gene, the protein is 455 amino acids in length and 50 kDa in size.
    基因ID
    4961525
    NCBI登录号
    YP_001129410
    UniProt
    Q2HR75
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