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POGZ 抗体 (N-Term)

Rockland 600-401-a89 POGZ 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN349593
Supplier Product No.: 600-401-a89
发货至: 中国
  • 抗原 See all POGZ 抗体
    POGZ (Pogo Transposable Element with ZNF Domain (POGZ))
    抗原表位
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    N-Term
    适用
    • 21
    • 12
    • 9
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    小鼠
    宿主
    • 20
    • 2
    克隆类型
    • 22
    多克隆
    标记
    • 18
    • 2
    • 1
    • 1
    This POGZ antibody is un-conjugated
    应用范围
    • 16
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    600-401-a89
    Supplier
    Rockland
    原理
    Pogz Antibody
    交叉反应 (详细)
    This antibody is known to react with mouse Pogz protein.
    产品特性
    Synonyms: rabbit anti-POGZ antibody, pogo transposable element with ZNF domain antibody, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635, SUHW5, ZNF280E, ZNF635
    纯化方法
    This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product POGZ Primary Antibody
  • 应用备注

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 155 kDa in size corresponding to Pogz by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:2,000 to 1:20,000

    ELISA Dilution: 1:100,000 - 1:400,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.6 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    POGZ (Pogo Transposable Element with ZNF Domain (POGZ))
    别名
    Pogz (POGZ 产品)
    背景
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors.  Mouse Pogz has a human homolog with a base pair similarity of 90 % and amino acid similarity of 93 % for the full length protein.  In humans at least 3 variants have been predicted and they are all similar at the N-terminus.   A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells.  In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.
    基因ID
    229584
    UniProt
    Q8BZH4
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