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Biotin 抗体

Rockland 800-101-098 WB, ELISA, IHC, DB 宿主: 山羊 Polyclonal unconjugated Fab fragment
Rockland
产品编号 ABIN349590
Supplier Product No.: 800-101-098
发货至: 中国
  • 抗原 See all Biotin products
    Biotin
    片段
    Fab fragment
    适用
    请咨询
    宿主
    • 62
    • 35
    • 29
    山羊
    克隆类型
    • 81
    • 37
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    多克隆
    标记
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    This Biotin antibody is un-conjugated
    应用范围
    • 75
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
    Supplier Product No.
    800-101-098
    Supplier
    Rockland
    原理
    Fab Biotin Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
    产品特性
    Synonyms: Goat Fab Anti-Biotin Antibody, Goat Fab Fragment Anti-Biotin Antibody
    纯化方法
    Fab fragment of Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Other

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Fab Anti-Biotin Antibody has been tested by ELISA and dot blot. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: User Optimized

    ELISA Dilution: 1:5,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    有效期
    12 months
  • 抗原
    Biotin
    Abstract
    Biotin 产品
    物质类
    Chemical
    背景
    Background: Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
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