EIF3E 抗体 (C-Term)
Quick Overview for EIF3E 抗体 (C-Term) (ABIN349583)
抗原
See all EIF3E 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-a62
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Supplier
- Rockland
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原理
- Eif3s6 Int6 Antibody
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交叉反应 （详细）
- This antibody is specific for mouse eIF3S6/Int6 protein.
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产品特性
- Synonyms: rabbit anti-Eif3s6/Int6 Antibody, rabbit anti-Eif3s6 Antibody, rabbit anti-Int6 Antibody, eIF3e antibody, Eukaryotic translation initiation factor 3 subunit 6 antibody, INT-6 antibody, Viral integration site protein INT 6 antibody, Mammary tumor-associated protein INT-6, MMTV integration site 6, Eukaryotic translation initiation factor 3 subunit E
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (Middle Region) antibody
EIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 346-445) antibodyEIF3E 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 1-445) antibodyEIF3E 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 36-85) antibodyEIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 248-276) antibodyEIF3E 适用: 人, 斑马鱼 WB 宿主: 兔 Polyclonal RB40515 unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (N-Term) antibodyEIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (C-Term) antibodyVerified EIF3E 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 360-409) antibodyEIF3E 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 兔, 猴, Pig, Bat, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) (AA 1-445) antibodyEIF3E 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E) antibodyEIF3E 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to eIF3S6/Int6 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous eIF3S6/Int6.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:90,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.8 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))
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别名
- Eif3e
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth. Anti-eIF3S6/Int6 Antibody is useful for researchers interested in cancer and apoptosis research.
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基因ID
- 16341, 45476573
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UniProt
- P60229
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途径
- Ribonucleoprotein Complex Subunit Organization, Hepatitis C
抗原
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