Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
特异性
The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
交叉反应 (详细)
Human, Other not determined
纯化方法
Purified by protein-A affinity chromatography.
纯度
> 95 % (by SDS-PAGE)
免疫原
KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line. Western blotting: The antigen migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
限制
仅限研究用
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Leukocyte specific transcript 1,LST1 (leukocyte-specific transcript 1, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A –, LST1/N) can be detected, some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.,LST-1, B144