CD4 抗体
(9 references)-
- 抗原 See all CD4 抗体
- CD4
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func)
- 特异性
- The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
- 交叉反应 （详细）
- Mouse
- 纯化方法
- Purified by protein-G affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Mouse CTL clone V4 cells
- 克隆位点
- GK1-5
- 亚型
- IgG2b
- Top Product
- Discover our top product CD4 Primary Antibody
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Pig, 犬, Cat, 兔, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠 IF, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 适用: 人 FACS, WB, CyTOF 宿主: 小鼠 Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) (AA 300-400) antibodyCD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE
anti-CD4 (CD4) antibody (PE)CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE
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- 应用备注
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Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
Immunocytochemistry: Recommended dilution: 1-4 μg/mL.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 mL lysate.
Flow cytometry: Recommended dilution: 1 μg/million cells.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4
- 储存液
- Azide free
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6593-603, (2008) (PubMed).
: "Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice." in: Transplantation, Vol. 85, Issue 8, pp. 1167-74, (2008) (PubMed).
: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, (2007) (PubMed).
: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).
: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, (1994) (PubMed).
: "IL-12 influences intrathymic T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2729-35, (1994) (PubMed).
: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).
: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, (1983) (PubMed).
: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, (1983) (PubMed).
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: "Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6593-603, (2008) (PubMed).
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- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 别名
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- 背景
- CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4
- 基因ID
- 12504
- UniProt
- P06332
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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