CD14 抗体 (AA 9-13, AA 39-44)
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- 抗原 See all CD14 抗体
- CD14
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抗原表位
- AA 9-13, AA 39-44
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD14 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), ELISA, Functional Studies (Func), Blocking Peptide (BP)
- 特异性
- Soluble and membrane bound human CD14. The antibody blocks LPS binding to membrane bound CD14
- 交叉反应
- Corynebacterium diphtheriae, 牛, 马
- 交叉反应 (详细)
- horse and bovine CD14,inhibits binding of LPS to horse and bovine CD14. Binding titre at horse CD14 1:100 and inhibition titre 1:10.
- 纯化方法
- Protein A affinity column
- 免疫原
- Monocytes of different species and immunoaffinity purified soluble human CD14
- 克隆位点
- BiG10
- 亚型
- IgG1
- Top Product
- Discover our top product CD14 Primary Antibody
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- 应用备注
- total inhibition of LPS-binding to CD14: 100 μg/mL. Binding titre at human CD14 transfected CHO-cells: more than 10.000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with bidistilled water
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH 7.4
- 储存液
- Without preservative
- 储存条件
- -20 °C,-80 °C
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Analysis of CD14 Expression Levels in Putative Mesenchymal Progenitor Cells Isolated from Equine Bone Marrow." in: Stem cells and development, (2010) (PubMed).
: "Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity." in: Veterinary immunology and immunopathology, Vol. 131, Issue 1-2, pp. 1-8, (2009) (PubMed).
: "The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms." in: European journal of biochemistry / FEBS, Vol. 236, Issue 2, pp. 457-64, (1996) (PubMed).
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Analysis of CD14 Expression Levels in Putative Mesenchymal Progenitor Cells Isolated from Equine Bone Marrow." in: Stem cells and development, (2010) (PubMed).
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- 抗原
- CD14
- 别名
- CD14 (CD14 产品)
- 别名
- CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody
- 背景
- Background: biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.
- 分子量
- ~50kDa
- 基因ID
- 929
- NCBI登录号
- NP_000582
- UniProt
- P08571
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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