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Myc Tag 抗体 (AA 410-420)

Name: Myc Tag 抗体 (AA 410-420)
SKU: ABIN339758
Availability: OutOfStock

WB, IP 宿主: 小鼠 Monoclonal unconjugated

产品编号 ABIN339758

发货至: 中国

Datasheet as PDF

抗原 See all Myc Tag products

Myc Tag
抗原表位
21

21

12

8

6

2

2

1
AA 410-420

适用

请咨询
宿主
75

49

6

5

5

5

1

1
小鼠
Compatible Secondaries
克隆类型
77

58

10
单克隆
标记
67

13

10

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is un-conjugated
应用范围
110

53

43

37

26

24

20

18

15

11

11

6

6

5

4

4

4

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP)

序列

EQKLISEEDL

特异性

Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

预测反应

Percent identity by BLAST analysis: Mouse (100%) Human, Chimpanzee, Gorilla, Rat, Amoeba (91%) Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).

纯化方法

Protein G purified

免疫原

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Percent identity by BLAST analysis: Mouse (100%), Human, Chimpanzee, Gorilla, Rat, Amoeba (91%), Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (82%).

Type of Immunogen: Synthetic peptide - KLH conjugated

亚型

IgG1
anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
适用: 人 FACS 宿主: 小鼠 Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF 宿主: 小鼠 Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB 宿主: 山羊 Polyclonal unconjugated

anti-Myc Tag (AA 408-439) antibody
适用: 人 WB, IP, IF, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 9E10 unconjugated Recombinant Antibody

应用备注

Approved: IP, WB

Usage: Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 μg/mL detects Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag. Lysates from NIH/3T3 cells transfected with either empty vector, PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLLRKRGST, or PP2A A subunit containing the Myc epitope tag sequence MEQKLISEEDLNGST were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Myc Tag (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Myc-tagged PP2A A subunit (~63kD). Longer exposure demonstrating weak detection by anti-Myc Tag of the Myc-tagged protein containing the epitope tag sequence MEQKLISEEDLNGST. Immunoprecipitation: Reported that this antibody immunoprecipitates Myc-tagged protein from transfected cells.

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
抗原

Myc Tag

Abstract

Myc Tag 产品

物质类

Tag