AKT Substrate (pSer), (pThr) 抗体
ELISA, WB, IHC (p), IP
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- AKT Substrate
- 抗原表位
- pSer, pThr
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 特异性
- Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.
- 纯化方法
- Immunoaffinity Chromatography
- 免疫原
- Synthetic phospho- Akt substrate peptides.
- 亚型
- IgG
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- 应用备注
- ELISA (1:1000), IHC-P, IP (1:20), WB (1:1000)
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.
- 注意事项
- avoid freeze thaw cycles. Store undiluted.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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- 抗原
- AKT Substrate
- 物质类
- Chemical
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