C5AR1 抗体
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- 抗原 See all C5AR1 抗体
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
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适用
- 人, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This C5AR1 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Neutralization (Neut)
- 品牌
- IHC-plus™
- 特异性
- Recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone 3H1738 was raised against a synthetic peptide Met1 -Asn31 and has recently been shown to recognize the heptameric peptide (D15DKDTLD21). Clone 3H1738 inhibits C5a binding to its receptor. Species cross-reactivity: Rabbit.
- 纯化方法
- Ion exchange chromatography
- 免疫原
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C5aR - peptide: Met1 - Asn31.
Type of Immunogen: Synthetic peptide - 亚型
- IgG2a
- Top Product
- Discover our top product C5AR1 Primary Antibody
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- 应用备注
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Approved: Flo (1:50), Func, IHC, IHC-P (10 μg/mL), Neut
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: 1:50. Use 20 μL to label 5x10^5 cells in 100 μL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 40 % glycerol. No preservative added.
- 储存液
- Azide free
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
- 别名
- C5AR1 / CD88 / C5a Receptor (C5AR1 产品)
- 别名
- C5A antibody, C5AR antibody, C5R1 antibody, CD88 antibody, C5aR antibody, C5r1 antibody, Cd88 antibody, D7Msu1 antibody, GPR77 antibody, complement C5a receptor 1 antibody, complement component 5a receptor 1 antibody, C5AR1 antibody, C5ar1 antibody, c5ar1 antibody
- 背景
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Name/Gene ID: C5AR1
Subfamily: Anaphylatoxin
Family: GPCR
Synonyms: C5AR1, C5A, C5a-R, C5a ligand, C5a receptor, CD88, C5a anaphylatoxin receptor, C5AR, C5R1, CD88 antigen - 基因ID
- 728
- UniProt
- P21730
- 途径
- Complement System
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