PAR1 抗体 (N-Term)
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- 抗原 See all PAR1 (F2R) 抗体
- PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PAR1 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 品牌
- IHC-plus™
- 特异性
- Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.
- 纯化方法
- Protein A purified
- 免疫原
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A synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.
Type of Immunogen: Synthetic peptide - 克隆位点
- ATAP2
- 亚型
- IgG1
- Top Product
- Discover our top product F2R Primary Antibody
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- 应用备注
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Approved: ELISA (0.1 - 1 μg/mL), Flo (10 - 20 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 μg/mL. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 μg/mL. ELISA: 0.1-1.0 μg/mL.
Not recommended for: WB - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.1 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))
- 别名
- F2R / Thrombin Receptor / PAR1 (F2R 产品)
- 别名
- CF2R antibody, HTR antibody, PAR-1 antibody, PAR1 antibody, TR antibody, Par1 antibody, TRGPC antibody, AI482343 antibody, Cf2r antibody, ThrR antibody, Par-1 antibody, f2r-a antibody, par1 antibody, coagulation factor II thrombin receptor antibody, coagulation factor II (thrombin) receptor antibody, coagulation factor 2 (thrombin) receptor L homeolog antibody, F2R antibody, F2r antibody, f2r.L antibody
- 背景
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Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR
Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1 - 基因ID
- 2149
- UniProt
- P25116
- 途径
- Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
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