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Complement C2 抗体

适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN337358
发货至: 中国
  • 抗原 See all Complement C2 抗体
    Complement C2
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This Complement C2 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Recognizes C2 from human plasma/serum. Epitope specificity differs from that of LS-C22277 and LS-C22278 as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2, when tested in sandwich ELISA with a polyclonal antibody against C2. In ELISA, reaction is also seen with C2 directly coated onto the microtiter well.
    纯化方法
    Protein A/G purified
    免疫原
    Complement component C2 isolated from human plasma.

    Type of Immunogen: Purified protein
    亚型
    IgG1 kappa
    Top Product
    Discover our top product Complement C2 Primary Antibody
  • 应用备注
    Approved: ELISA (1:2000), IHC, IHC-P (10 μg/mL), WB (1:125)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原
    Complement C2
    Abstract
    Complement C2 产品
    别名
    CO2 antibody, complement C2 antibody, complement component 2 (within H-2S) antibody, C2 antibody
    背景
    Name/Gene ID: C2
    Subfamily: Serine S1
    Family: Protease

    Synonyms: C2, C2b, C3/C5 convertase, Complement component C2, Complement C2, Complement component 2, CO2
    基因ID
    717
    UniProt
    P06681
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