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PRAS40 抗体

AKT1S1 适用: 人 WB, IHC, IHC (p), IP 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN337263
发货至: 中国
  • 抗原 See all PRAS40 (AKT1S1) 抗体
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This PRAS40 antibody is un-conjugated
    应用范围
    • 63
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    品牌
    IHC-plus™
    特异性
    Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.
    纯化方法
    Protein A/G purified
    免疫原
    Recombinant full length human PRAS40 expressed in E. coli.

    Type of Immunogen: Recombinant protein
    亚型
    IgG1 kappa
    Top Product
    Discover our top product AKT1S1 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (10 μg/mL), IP, WB (0.1 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 μg/mL. Positive control: HeLa cells, Jurkat cells.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.2, 0.1 % sodium azide, 1 % BSA.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    别名
    AKT1S1 / PRAS40 (AKT1S1 产品)
    别名
    MGC81452 antibody, lobe antibody, pras40 antibody, fb34a04 antibody, wu:fb34a04 antibody, Lobe antibody, PRAS40 antibody, 1110012J22Rik antibody, AI227026 antibody, Lobel antibody, AKT1 substrate 1 (proline rich) S homeolog antibody, AKT1 substrate 1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 (proline-rich) antibody, akt1s1.S antibody, AKT1S1 antibody, akt1s1 antibody, Akt1s1 antibody
    背景
    Name/Gene ID: AKT1S1

    Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, Lobe
    基因ID
    84335
    UniProt
    Q96B36
    途径
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
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