STAT5 A/B 抗体 (pTyr694)
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- 抗原 See all STAT5 A/B products
- STAT5 A/B
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抗原表位
- pTyr694
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This STAT5 A/B antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100 % homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.
Type of Immunogen: Synthetic peptide
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- 应用备注
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Approved: ELISA (1:25000), IHC, IHC-P (20 - 40 μg/mL), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20-40 μg/mL. Western Blot: Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein in the appropriate cell lysate or extract. This phosphospecific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- 抗原
- STAT5 A/B
- 别名
- STAT5A+B (STAT5 A/B 产品)
- 背景
- This antibody detects: STAT5 A+B
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