IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) Dilute conc. antibodies 1:50-1:100 using streptavidin-Biotin system or polymer system for 30 minutes at RT. Prediluted antibody is used directly without dilution. The optimum dilution should be determined by the individual lab. Positive Control: Human gastrointestinal tumor. Cellular Localization: Cytoplasmic and epithelial membrane.
限制
仅限研究用
状态
Liquid
缓冲液
The antibodies are supplied as concentrated cell culture medium in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Aberrant glycosylation is a hallmark of cancer cells. The blood group precursors T (Thomsen-Friedenreich) and Tn epitopes are shielded in healthy and benign-diseased tissues but uncovered in approx. 90% of carcinomas. T and Tn glycoproteins are specific, autoimmunogenic pancarcinoma antigens. These antigens may also be found in neoplastic blood cells Fundamental chemical and physical aspects of these glycoproteins of primary carcinomas are discussed first. Tn and T epitopes are cell and tissue adhesion molecules, essential in invasion by and metastasis of carcinoma which includes adherent and proliferative phases. Immunohistochemical studies of the extent of Tn antigen expression in primary breast carcinoma demonstrate it highly significant correlation with clinicopathological tumor stages, and hence its value as a reliable prognosticator. This antibody stains Tn antigen in epithelial carcinoma, pancreatic, colon, stomach, breast.