ELISA (1/100 and 1/600). Has been used in a simple ELISA using 1 g of antigen coated perwell, using alkaline phosphatase conjugated anti-sheep for detection. All incubations at18C using pNPP substrate, with OD measurements at 405 nm. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
20 mg/mL
缓冲液
PBS, pH 7.2 without preservatives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.