Selectin E/CD62e 抗体
(9 references)-
- 抗原 See all Selectin E/CD62e (SELE) 抗体
- Selectin E/CD62e (SELE) (Selectin E (SELE))
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适用
- 人, 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Selectin E/CD62e antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
- 特异性
- UZ4 recognizes the minimal amino acid consensus sequence CXKKKL present in the lectin domain of both murine and human E-selectin. UZ4 is a functional antibody in that it inhibits leukocyte adhesion to both murine and human activated endothelial cells. For in vivo application in mice 20µg of purified Ig per injection is recommended.
- 纯化方法
- Purified
- 免疫原
- UZ4 is a rat monoclonal IgM antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
- 克隆位点
- UZ4
- 亚型
- IgM
- Top Product
- Discover our top product SELE Primary Antibody
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anti-Selectin E (SELE) (AA 501-612) antibody
SELE 适用: 人, Pig, 小鼠, 大鼠 WB, ELISA, FACS, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (AA 22-230) antibodySELE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (N-Term) antibodySELE 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (AA 108-238) antibodySELE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (AA 260-420) antibodySELE 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) antibodySELE 适用: 小鼠 WB, IHC, ELISA, FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal UZ5 unconjugated
anti-Selectin E (SELE) antibodySELE 适用: 人 ELISA 宿主: 小鼠 Monoclonal B-P7 unconjugated
anti-Selectin E (SELE) (AA 81-130) antibodySELE 适用: 人, Pig, 小鼠, 猴, 豚鼠, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Selectin E (SELE) (AA 1-610) antibodySELE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- UZ4 is also suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry (dilution 1:500). It is not optimal for immunohistochemistry; to analyze mouse E-selectin protein in frozen sections, please refer to the anti Eselectin monoclonal antibodies UZ5, UZ6, and UZ7.
- 限制
- 仅限研究用
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- 浓度
- 1mg/ml
- 缓冲液
- Purified rat monoclonal antibody at a concentration of 1mg/ml in PBS without azide!
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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: "Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation." in: Blood, Vol. 114, Issue 8, pp. 1696-706, (2009) (PubMed).
: "Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion." in: Experimental cell research, Vol. 269, Issue 2, pp. 266-74, (2001) (PubMed).
: "TNF-alpha-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 2, pp. 1300-7, (2001) (PubMed).
: "Different pathways leading to cutaneous leukocytoclastic vasculitis in mice." in: Experimental dermatology, Vol. 10, Issue 6, pp. 391-404, (2001) (PubMed).
: "Control of neonatal tolerance to tissue antigens by peripheral T cell trafficking." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1338-41, (1998) (PubMed).
: "CD4+ T cells migrate into inflamed skin only if they express ligands for E- and P-selectin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 2, pp. 963-70, (1998) (PubMed).
: "P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues." in: Nature, Vol. 385, Issue 6611, pp. 81-3, (1997) (PubMed).
: "P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 573-8, (1997) (PubMed).
: "Adhesion of leukocytes to the inflammed endothelium." in: Scandinavian journal of rheumatology. Supplement, Vol. 101, pp. 107-9, (1995) (PubMed).
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: "Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation." in: Blood, Vol. 114, Issue 8, pp. 1696-706, (2009) (PubMed).
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- 抗原
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- 别名
- E-selectin (SELE 产品)
- 别名
- SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody
- 背景
- BACKGROUND Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFα and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFκB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
- 途径
- Thromboxane A2 Receptor Signaling
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