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Vimentin 抗体

This anti-Vimentin antibody is a 小鼠 单克隆 antibody detecting Vimentin in WB, IHC, FACS, ICC 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, 小鸡, 猴, 犬, 山羊, Hamster 和 斑马鱼. This Primary Antibody has been cited in 5+ publications.
产品编号 ABIN335380
发货至: 中国

Quick Overview for Vimentin 抗体 (ABIN335380)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, 小鼠, 大鼠, 小鸡, 猴, 犬, 山羊, Hamster, 斑马鱼

宿主

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小鼠

克隆类型

  • 202
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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

RV202
  • 特异性

    Human, mouse, rat, hamster, chicken, goat, canine and monkey.

    纯化方法

    Purified

    免疫原

    RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

    亚型

    IgG1
  • 应用备注

    RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

    Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

    Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

    Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

    Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

    途径

    Caspase Cascade in Apoptosis
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