NEFH 抗体
-
- 抗原 See all NEFH 抗体
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
-
适用
- 人, 大鼠, 小鼠, 小鸡, Cow, 豚鼠, 兔, 犬, 绵羊, 非洲爪蟾, 猴, Hamster
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This NEFH antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Human, rat, rabbit, dog, hamster, chicken, mouse, cow, monkey, sheep, guinea pig and Xenopus.
- 纯化方法
- Purified
- 免疫原
- RNF402 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a neurofilament preparation of calf brain tissue.
- 克隆位点
- RNF402
- 亚型
- IgM
- Top Product
- Discover our top product NEFH Primary Antibody
-
-
- 应用备注
- RNF402 reacts with both the phosphorylated and non-phosphorylated isoform of the 200 kD neurofilament protein. RNF402 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:10 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
-
-
Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, (1992) (PubMed).
: "Expression of intermediate filament proteins in the mature inner ear of the rat and guinea pig." in: Hearing research, Vol. 52, Issue 1, pp. 133-46, (1991) (PubMed).
: "
-
Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, (1992) (PubMed).
-
- 抗原
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
- 别名
- Neurofilament 200 kDa (NEFH 产品)
- 别名
- NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody
- 背景
- Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
-