Plakophilin 3 抗体
(1 reference)-
- 抗原 See all Plakophilin 3 (PKP3) 抗体
- Plakophilin 3 (PKP3)
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适用
- 人, 小鼠, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Plakophilin 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Human, mouse and dog.
- 纯化方法
- Purified
- 免疫原
- 23E3/4 is a mouse monoclonal IgG2b antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
- 克隆位点
- 23E3-4
- 亚型
- IgG2b
- Top Product
- Discover our top product PKP3 Primary Antibody
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anti-Plakophilin 3 (PKP3) (AA 225-324) antibody
PKP3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6E5 unconjugated
anti-Plakophilin 3 (PKP3) (C-Term) antibodyPKP3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 3 (PKP3) (AA 225-324) antibodyPKP3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Plakophilin 3 (PKP3) (AA 1-308) antibodyPKP3 适用: 人, 小鼠 WB, IP, IHC (fro), HCP 宿主: 小鼠 Monoclonal 7F6 unconjugated
anti-Plakophilin 3 (PKP3) (AA 1-797) antibodyPKP3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Plakophilin 3 (PKP3) (AA 570-589) antibodyPKP3 适用: 人, 小鼠, 犬 WB, IHC, IP, IHC (fro), ICC, IHC (p) 宿主: 小鼠 Monoclonal 23E3-4 unconjugated
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- 应用备注
- 23E3/4 reacts with an epitope located between residues 570-589 in human plakophilin-3. 23E3/4 is suitable for immunocytochemistry (methanol- and paraformaldehyde-fixed cells), immunohistochemistry on frozen and paraffin-embedded tissues, immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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: "Plakophilin-3, a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells." in: Journal of cell science, Vol. 112 ( Pt 14), pp. 2265-76, (1999) (PubMed).
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: "Plakophilin-3, a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells." in: Journal of cell science, Vol. 112 ( Pt 14), pp. 2265-76, (1999) (PubMed).
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- 抗原
- Plakophilin 3 (PKP3)
- 别名
- Plakophilin-3 (PKP3 产品)
- 别名
- wu:fa97f09 antibody, wu:fk69a12 antibody, zgc:136656 antibody, PKP3 antibody, 2310056L12Rik antibody, plakophilin 3a antibody, plakophilin 3 antibody, plakophilin-3 antibody, plakophilin 3 L homeolog antibody, pkp3a antibody, PKP3 antibody, LOC450921 antibody, pkp3.L antibody, Pkp3 antibody
- 背景
- Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
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