S100A9 抗体 (Internal Region)
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- 抗原 See all S100A9 抗体
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This S100A9 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- S100A9
- 序列
- DTNADKQLSF EEF
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- C-DTNADKQLSFEEF
- 亚型
- IgG
- Top Product
- Discover our top product S100A9 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 13-14 kDa band observed in Human Bone Marrow lysates and approx 14-15 kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2 kDa according to NP_002956.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour a
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended conc
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
- 别名
- S100A9 (S100A9 产品)
- 背景
- S100A9, S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B
- 基因ID
- 6280
- NCBI登录号
- NP_002956
- 途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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