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EWSR1 抗体 (Internal Region)

Verified EWSR1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN335135
发货至: 中国
  • 抗原 See all EWSR1 抗体
    EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))
    抗原表位
    • 13
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 74
    • 35
    • 29
    • 11
    • 8
    • 8
    • 7
    • 7
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    宿主
    • 63
    • 10
    • 1
    • 1
    山羊
    克隆类型
    • 66
    • 9
    多克隆
    标记
    • 52
    • 5
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EWSR1 antibody is un-conjugated
    应用范围
    • 65
    • 31
    • 14
    • 11
    • 7
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    原理
    EWS / EWSR1
    序列
    TSYDQSSYSQ QNTYG
    特异性
    This antibody is expected to recognize all five reported isoforms (NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1).
    交叉反应
    Cow, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-TSYDQSSYSQQNTYG, from the internal region of the protein sequence according to NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1.
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5-7 μg/mL.

    Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 68.5 kDa according to NP_053733.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))
    别名
    EWSR1 (EWSR1 产品)
    别名
    EWS antibody, bK984G1.4 antibody, AU018891 antibody, Ews antibody, Ewsh antibody, EWSR1 antibody, fc04c01 antibody, wu:fc04c01 antibody, DKFZp459K1116 antibody, ewsr1 antibody, ewsr1.S antibody, fb40b11 antibody, fusl antibody, wu:fb40b11 antibody, wu:fb75g09 antibody, zgc:55864 antibody, EWS RNA binding protein 1 antibody, Ewing sarcoma breakpoint region 1 antibody, EWS RNA-binding protein 1 antibody, EWS RNA-binding protein 1a antibody, EWS RNA binding protein 1 L homeolog antibody, EWS RNA-binding protein 1b antibody, EWSR1 antibody, Ewsr1 antibody, ewsr1a antibody, ewsr1 antibody, ewsr1.L antibody, ewsr1b antibody
    背景
    EWSR1, EWS, Ewing sarcoma breakpoint region 1, EWS/FLI fusion protein, EWSR1/ZNF384 fusion protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, bK984G1.4 (Ewing sarcoma breakpoint region 1 protein), extraskeletal myxoid chondrosarcoma EWS/TEC/CHN fusion pr
    基因ID
    2130, 14030, 289752
    NCBI登录号
    NP_053733, NP_005234, NP_001156757, NP_001156758, NP_001156759
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