TOP2 抗体 (C-Term, N-Term)
(1 reference)-
- 抗原 See all TOP2 抗体
- TOP2 (DNA Topoisomerase II (TOP2))
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抗原表位
- C-Term, N-Term
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适用
- Arabidopsis thaliana, Vicia faba
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TOP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunolocalization (IL)
- 特异性
- Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.
- 交叉反应 （详细）
- Not reactive in: Nicotiana tabacum
- 预测反应
- Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)
- 纯化方法
- serum
- 免疫原
- The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.
- Top Product
- Discover our top product TOP2 Primary Antibody
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anti-DNA Topoisomerase II (TOP2) (AA 923-1148) antibody
TOP2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Topoisomerase II (TOP2) (AA 1079-1328) antibodyTOP2 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Topoisomerase II (TOP2) (AA 749-987) antibodyTOP2 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- 1: 2000 (WB), 1: 500 (IL)
- 说明
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Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 200 μL of sterile water
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).
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: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).
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- 抗原
- TOP2 (DNA Topoisomerase II (TOP2))
- 别名
- TOP2 (TOP2 产品)
- 背景
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AGI Code: At3g23890
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II - 分子量
- expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)
- UniProt
- P30182
- 途径
- Mitotic G1-G1/S Phases
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