COXII 抗体
COXII
适用: Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum, Zea mays
WB, BN PAGE
宿主: 兔
Polyclonal
unconjugated
1 image
(4 references)-
- 抗原 See all COXII products
- COXII (Cytochrome Oxidase Subunit II (COXII))
- 适用
- Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum, Zea mays
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COXII antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Blue-native PAGE (BN PAGE)
- 特异性
- Arabidopsis thaliana (leaf extract and isolated mitochondria).
- 预测反应
- dicots including Cucumis melo, Glycine max, Pisum sativum, monocots including Oryza sativa, moss Physcomitrella patens
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 29.4 / 30 kDa (for Arabidopsis thaliana)
- 纯化方法
- serum
- 免疫原
- KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9
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- 应用备注
- Recommended Dilution 1 : 1000 (WB), 1 : 1000 (BN-PAGE).
- 说明
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antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).
: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).
: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." in: The New phytologist, (2010) (PubMed).
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: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
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- 抗原
- COXII (Cytochrome Oxidase Subunit II (COXII))
- 别名
- COXII (COXII 产品)
- 别名
- cydB antibody, SCD78.13 antibody, qxtB antibody, cytochrome oxidase subunit II antibody, cytochrome d ubiquinol oxidase subunit II antibody, ubiquinol oxidase subunit II, cyanide insensitive antibody, COXII antibody, SCO3946 antibody, STM1793 antibody, cioB antibody, ctaC antibody, BPSS0234 antibody, appB antibody, BURPS1106A_A1873 antibody, PMI_RS06945 antibody, SSPA1008 antibody, SG1323 antibody, SEN1244 antibody, Sros_6663 antibody, VDBG_17011 antibody
- 背景
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AGI Code: ATMG00160
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2 - 分子量
- expected: 29.4 kDa, apparent: 30 kDa (for Arabidopsis thaliana)
- UniProt
- Q1XGA9, P04373
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