PRPF31 抗体 (Internal Region)
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- 抗原 See all PRPF31 抗体
- PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PRPF31 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- PRPF31
- 序列
- KELGNSLDKC KNNEN
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence KELGNSLDKCKNNEN, from the internal region of the protein sequence according to NP_056444.3.
- 亚型
- IgG
- Top Product
- Discover our top product PRPF31 Primary Antibody
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- 应用备注
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Western Blot: Approx 60 kDa band observed in nuclear lysates of cell lines A431, HeLa, HEK293 and NIH3T3 (calculated MW of 55.4 kDa according to Human NP_056444.3 and Mouse NP_081604.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at roo
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))
- 别名
- PRPF31 (PRPF31 产品)
- 别名
- DDBDRAFT_0219465 antibody, DDBDRAFT_0304959 antibody, DDB_0219465 antibody, DDB_0304959 antibody, PRPF31 antibody, NY-BR-99 antibody, PRP31 antibody, RP11 antibody, SNRNP61 antibody, prp31 antibody, rp11 antibody, 1500019O16Rik antibody, 2810404O06Rik antibody, AW554706 antibody, zgc:66177 antibody, pre-mRNA processing factor 31 antibody, PrpF31 U4/U6*U5 snRNP-associated pre-mRNA processing factor 31, (IC) antibody, U4/U6 small nuclear ribonucleoprotein Prp31 antibody, pre-mRNA processing factor 31 S homeolog antibody, PRP31 pre-mRNA processing factor 31 homolog (yeast) antibody, PRPF31 antibody, prpf31 antibody, PrpF31 antibody, LOC100455616 antibody, Prpf31 antibody, prpf31.S antibody
- 背景
- PRPF31, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), DKFZp566J153, NY-BR-99, PRP31, RP11 , PRP31 pre-mRNA processing factor 31 homolog (yeast), pre-mRNA processing factor 31 homolog, pre-mRNA processing factor 31 homolog (yeast)
- 基因ID
- 26121, 68988
- NCBI登录号
- NP_056444
- 途径
- Ribonucleoprotein Complex Subunit Organization
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