ITCH 抗体 (Internal Region)
-
- 抗原 See all ITCH 抗体
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
-
抗原表位
- Internal Region
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This ITCH antibody is un-conjugated
-
应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- ITCH / AIF4
- 序列
- EIKSHDLKPN GGN
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
- 亚型
- IgG
-
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibody
KO Validated ITCH 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (Tyr420) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 29-303) antibodyITCH 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) antibodyITCH 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 50-150) antibodyKO Validated ITCH 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Monoclonal 9C3 unconjugated Recombinant Antibody
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (pTyr420) antibodyITCH 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 143-192) antibodyITCH 适用: 人, Cow, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 410-437), (Middle Region) antibodyITCH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- 别名
- ITCH (ITCH 产品)
- 别名
- AIF4 antibody, AIP4 antibody, NAPP1 antibody, dJ468O1.1 antibody, 6720481N21Rik antibody, 8030492O04Rik antibody, A130065M08 antibody, C230047C07Rik antibody, itchy E3 ubiquitin protein ligase antibody, itchy, E3 ubiquitin protein ligase antibody, ITCH antibody, Itch antibody
- 背景
- ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui
- 基因ID
- 83737, 16396, 311567
- NCBI登录号
- NP_113671, NP_001244066, NP_001244067
- 途径
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
-
