alpha 2 Antiplasmin 抗体 (Internal Region)
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- 抗原 See all alpha 2 Antiplasmin (SERPINF2) 抗体
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This alpha 2 Antiplasmin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Alpha2-antiplasmin
- 序列
- KDFLQSLKGF PRGDK
- 特异性
- This antibody is expected to recognise both reported isoforms (NP_000925.2, NP_001159393.1). Reported variants represent identical protein (NP_000925.2, NP_001159392.1).
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2, NP_001159393.1.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5 μg/mL.
Western Blot: Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at r
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).
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On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).
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- 抗原
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
- 别名
- SERPINF2 (SERPINF2 产品)
- 别名
- A2AP antibody, AAP antibody, ALPHA-2-PI antibody, API antibody, PLI antibody, serpin antibody, AI747498 antibody, Pli antibody, Serpimf2 antibody, serpinf2 antibody, serpinf2l antibody, wu:fd57d08 antibody, Dmrs91 antibody, Pedf antibody, serpin family F member 2 antibody, serpin family F member 1 antibody, serine (or cysteine) peptidase inhibitor, clade F, member 2 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a antibody, SERPINF2 antibody, Serpinf2 antibody, SERPINF1 antibody, serpinf2a antibody, Serpinf1 antibody
- 背景
- SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment
- 基因ID
- 5345
- NCBI登录号
- NP_000925, NP_001159393
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
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