FAS 抗体 (Extracellular Domain) (PE)
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- 抗原 See all FAS 抗体
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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抗原表位
- Extracellular Domain
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适用
- Cat
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This FAS antibody is conjugated to PE
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), ELISA
- 特异性
- Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas.
- 纯化方法
- Protein G purified
- 免疫原
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NS0-derived recombinant feline rfeFas extracellular domain.
Type of Immunogen: Recombinant protein - 亚型
- IgG1
- Top Product
- Discover our top product FAS Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Cat
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
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- 抗原
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- 别名
- CD95 / FAS (FAS 产品)
- 别名
- ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
- 背景
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Name/Gene ID: FAS
Family: TNF Receptor
Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95 - 基因ID
- 355
- UniProt
- P25445
- 途径
- p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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