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CD28 抗体 (PE)

CD28 适用: 小鼠 FACS 宿主: 小鼠 Monoclonal E18 PE
产品编号 ABIN320280
发货至: 中国
  • 抗原 See all CD28 抗体
    CD28
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    小鼠
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    小鼠
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    标记
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    This CD28 antibody is conjugated to PE
    应用范围
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    Flow Cytometry (FACS)
    纯化方法
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    免疫原
    Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig. Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.
    克隆位点
    E18
    亚型
    IgG2b
    Top Product
    Discover our top product CD28 Primary Antibody
  • 应用备注
    Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 1 mL distilled water.
    浓度
    0.1 mg/mL
    缓冲液
    Phosphate buffered saline pH 7.4 without preservatives.
    储存液
    Without preservative
    注意事项
    This product is photosensitive and should be protected from light
    储存条件
    4 °C
    储存方法
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • 抗原
    CD28
    别名
    CD28 (CD28 产品)
    背景
    Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
    基因ID
    10090
    UniProt
    P31041
    途径
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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