This antibody recognises the alpha-chain of the CD8 antigen, a 32 kDa glycoprotein expressed by the cytotoxicsuppressor subset of T cells and weakly by NK cells.
纯化方法
Ion Exchange Chromatography
免疫原
Normal human peripheral blood lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL human wholeblood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1 mL distilled water
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Prior to and following reconstitution store the antibody at -20 °C.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2