V5 Epitope Tag 抗体
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- 抗原 See all V5 Epitope Tag products
- V5 Epitope Tag
- 适用
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This V5 Epitope Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)
- 序列
- GKPIPNPLLG LDST
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 免疫原
- Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
- 克隆位点
- SV5-Pk1
- 亚型
- IgG2a
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- 应用备注
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ELISA (1/1000-1/5000). Western Blot (1/1000-1/5000). Immunoprecipitation. Radioimmunoassays. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is manufactued under an exclusive license from the University of St. Andrews,UK. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- V5 Epitope Tag
- 别名
- Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag 产品)
- 物质类
- Tag
- 背景
- The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
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