CD86 抗体
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- 抗原 See all CD86 抗体
- CD86
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD86 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognises CD86. Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, Clone Bu63 is located within the Ig-v-like domain of Human CD86 (Jeanin et al. 1997).
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Human peripheral blood lymphocytes. Spleen cells from immunised mice were fused with cells of the P3 X63 Ag8.653 myeloma cell line.
- 克隆位点
- BU63
- 亚型
- IgG1
- Top Product
- Discover our top product CD86 Primary Antibody
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- 应用备注
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Immunoprecipitation. Flow Cytometry: 1/20-1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/20-1/100. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.1 M Tris buffered saline, pH 8.2, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD86
- 别名
- CD86 (CD86 产品)
- 别名
- B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody
- 背景
- CD86 is a 70kD cell surface protein expressed by activated B cells and monocytes. CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in costimulation of T cells in the primary immune response.Synonyms: Activation B7-2 antigen, B7.2, B70, BU63, CD28LG2, CTLA-4 counter-receptor B7.2, FUN-1, T-lymphocyte activation antigen CD86
- 基因ID
- 942
- NCBI登录号
- NP_008820
- UniProt
- P42081
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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