Isocitrate Dehydrogenase 抗体
IDH
适用: Arabidopsis thaliana, Pisum sativum, Potato, Tomato, Zea mays
WB
宿主: 兔
Polyclonal
unconjugated
1 image
(3 references)-
- 抗原 See all Isocitrate Dehydrogenase (IDH) 抗体
- Isocitrate Dehydrogenase (IDH)
- 适用
- Arabidopsis thaliana, Pisum sativum, Potato, Tomato, Zea mays
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Isocitrate Dehydrogenase antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 交叉反应 （详细）
- No cross-reactivity with: Chlamydomonas reinhardtii
- 预测反应
- dicots including Brassica napus, Vitis vinifera, monocots including Oryza sativa, Zea mays
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 39 / 45 kDa (Arabidopsis thaliana)
- 纯化方法
- serum
- 免疫原
- KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At4g17130
- Top Product
- Discover our top product IDH Primary Antibody
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anti-Isocitrate Dehydrogenase (IDH) antibody
IDH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase (IDH) (Middle Region) antibodyIDH 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Recommended Dilution 1 : 5 000 with standard ECL (WB). Additional Information: Peptide used to elicit this antibody is not conserved in NADPH dependentanzymes, partially conserved across eukaryotic Idh subunits. Some conservationacross bacterial which contain the NAD-dependent form of Idh (as opposed to theNADP-dependent form).
- 说明
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cellular [compartment marker] of mitochondrial matrix
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." in: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).
: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).
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: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
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- 抗原
- Isocitrate Dehydrogenase (IDH)
- Abstract
- IDH 产品
- 别名
- CG7176 antibody, CT22171 antibody, Dmel\\CG7176 antibody, IDH antibody, IDH-NADP antibody, Idh-NADP antibody, idh antibody, isocitrate dehydrogenase antibody, l(3)L3852 antibody, F20D21.16 antibody, F20D21_16 antibody, SMU.672 antibody, icd antibody, Isocitrate dehydrogenase antibody, isocitrate dehydrogenase antibody, isocitrate dehydrogenase, NADP-dependent antibody, Monomeric isocitrate dehydrogenase antibody, ankyrin repeat domain 26-like antibody, Idh antibody, ICDH antibody, idh antibody, icd antibody, SSO_RS10660 antibody, JK_RS08640 antibody, LOC618625 antibody
- 背景
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AGI Code: At4g35260
Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent. - 分子量
- expected: 39 kDa, apparent: 45 kDa (Arabidopsis thaliana)
- UniProt
- P93032
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