CD33 抗体
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- 抗原 See all CD33 抗体
- CD33
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD33 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- 特异性
- Recognizes the CD33 cell surface glycoprotein, a 67 kDa molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.
- 交叉反应 (详细)
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- Human AML cells
- 克隆位点
- WM53
- 亚型
- IgG1
- Top Product
- Discover our top product CD33 Primary Antibody
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- 应用备注
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Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections (1/50-1/100): Overnight incubation recommended. Positive control tissue: lymphatic tissue. Not suitable on Paraffin Embedded Sections. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD33
- 别名
- CD33 / SIGLEC3 (CD33 产品)
- 别名
- SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody
- 背景
- CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
- 基因ID
- 945
- NCBI登录号
- NP_001763
- UniProt
- P20138
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