Phospho-eNOS antibody (AP08011PU) detects endogenous levels of eNOS only when phosphorylated at serine 1177.
纯化方法
Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of Serine 1177 (T-Q-Sp-F-S).
Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Embedded Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ENOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial