Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody recognises TACR3 otherwise known as NK3 Receptor (NK3R), a 52.2 kDa multi-pass membrane protein belonging to the G-protein coupled receptor 1 family. This antibody does not recognise NK1R or NK2R.
纯化方法
Purified
免疫原
An 18 amino acid peptide from near the centre of Human TACR3.
TACR3
适用: 人
WB, IF, IC
宿主: 兔
Polyclonal
unconjugated
应用备注
Immunohistochemistry on paraffin sections: 5g/mL, requires antigen retrieval using heattreatment prior to staining of paraffin sections. Western Blot: 0.5 - 1.0g/mL, detects a band of approximately 46 kDa in RAW264.7 celllysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, 0.02 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.
TACR3 is a receptor for the tachykinin neuropeptide, neuromedin-k otherwise known as neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system, and it is likely that these signals lead to the release of vasopressin and oxytocin into the circulation. Studies with mice indicate that TACR3 is involved in learning and memory.Synonyms: NK-3 receptor, NK-3R, NK3R, NKR, Neurokinin B receptor, Neuromedin-K receptor, TAC3R