This antibody detects Apoptosis Signal-regulating Kinase 1 (ASK1) phosphorylated at serine83. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.
纯化方法
Purified
免疫原
KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
ELISA: 1: 5,000 - 1: 10,000. Western Blot: 1: 1,000, detects a band of approximately 155 kDa in SW1353 cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
ASK1 is a serine/threonine kinase also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5