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CD200 抗体 (Internal Region)

CD200 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3187966
发货至: 中国
  • 抗原 See all CD200 抗体
    CD200
    抗原表位
    • 15
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    • 3
    • 2
    • 2
    • 1
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    Internal Region
    适用
    • 92
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    • 2
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    • 2
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    宿主
    • 57
    • 44
    • 12
    克隆类型
    • 59
    • 52
    多克隆
    标记
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    This CD200 antibody is un-conjugated
    应用范围
    • 53
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    • 25
    • 16
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    • 8
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    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    CD200 Polyclonal Antibody detects endogenous levels of CD200 protein.
    产品特性
    Rabbit Polyclonal to CD200.
    纯化方法
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    免疫原
    Synthesized peptide derived from the Internal region of human CD200 (aa 171-220)
    亚型
    IgG
  • 应用备注
    WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000,
    限制
    仅限研究用
  • 生效 #103457 (Immunohistochemistry)
    '独立验证'标志
    by
    David A. Clark, Department of Medicine and Department of Pathology and Molecular Medicine, McMaster University
    No.
    #103457
    日期
    2018.11.30
    抗原
    CD200
    Lot Number
    6839401
    Method validated
    Immunohistochemistry
    Positive Control

    Human 7 week elective termination placenta

    Negative Control

    KLH antibody ABIN401183

    Diluent

    Notes

    Passed. The CD200 antibody ABIN318966 specifically recognizes its antigen in human placental tissue.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3187966
    Secondary Antibody
    Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232)
    Full Protocol
    • Fix human placental tissue in 10% buffered formalin for 24h at RT (22-23°C), process, and embedded in paraffin.
    • Cut paraffin blocks with a Leica CM2255 Microtome into 4µm sections.
    • Affix sections to positively charged slides and air dry ON at RT.
    • Dewax the slides and hydrate on the automated Leica BOND Rx stainer.
    • Antigen retrieval on a Leica BOND RX automated stainer using epitope retrieval buffer 2 (Leica, AR9640, lot ER20172).
    • Stain slides with primary
      • rabbit anti- CD200 antibody (AA 45-95) (Antibodies-online, ABIN761396, lot 980502W),
      • rabbit anti-CD200 antibody (AA 170-220) (Antibodies-online, ABIN318966 lot 6839401) (AA 170-220), or
      • rabbit anti-KLH antibody (Antibodies-online, ABIN401183, lot 304770)
    • diluted 1:200 in Power Vision IHC Super Blocker (Leica, PV6122). The staining protocol incorporates a modified Leica standard protocol IHC-F (which omits the post-primary step) and uses the standard times outlined in the machine protocol.
    • Stain sections with Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232) containing peroxidase block, post primary antibody, polymer as well as DAB chromogen and hematoxylin counterstain for times outlined in the standard protocol IHC-F.
    • Remove slides from the Leica Bond Rx and then dehydrate in ethanol and clear in xylene.
    • Mount slides mounted in Fisher Chemical Permount Mounting Medium (Fisher Scientific, SP15-500, lot 162767).
    • Once the slide-coverslip edges are dry, scan the slides using Leica Imagescope (40x) and photograph into jpg files at 400x.
    Experimental Notes
    • Several dilutions of ABIN318966 were tested and optimal staining was obtained at a dilution as low as 1:200.

    • The staining pattern of ABIN318966 differs from our result using ABIN761396 which matched RB846 and observations made by others. The result may be explainable by ABIN318966 reacting with variants of CD200 which lack upstream exon 3 coded AA that are eliminated along with exon 2 coded AA by alternative splicing so as to produce the truncated form of CD200 (CD200tr). Removing exon 2 + some exon 3 coded AA may expose epitopes in the downstream exon 3 + exon 4 region of CD200tr that are detected by antibody to the 170-220 AA region of CD200 that are in exon 4 of CD200 variant 1.

    • ABIN761396 stained villus trophoblasts as described in Clark et al. (2017).

    • ABIN761396 stained villus cytotrophoblasts and syncytiotrophoblasts but ABIN3187966 did not show convincing staining.

  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freeze/thaw cycles.
    储存条件
    -20 °C
    储存方法
    Store at -20°C.
  • Clark, Reihani, Arredondo, Ask, Foster: "CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells." in: Journal of reproductive immunology, Vol. 150, pp. 103477, (2022) (PubMed).

  • 抗原
    CD200
    别名
    CD200 (CD200 产品)
    别名
    MOX1 antibody, MOX2 antibody, MRC antibody, OX-2 antibody, CD200 antibody, Cspmo2 antibody, MRCOX2 antibody, Mox2 antibody, DKFZp459C0716 antibody, OX2 antibody, CD200 molecule antibody, Cd200 molecule antibody, CD200 antigen antibody, CD200 antibody, Cd200 antibody
    分子量
    31 kDa
    基因ID
    4345
    UniProt
    P41217
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