This antibody detects Slug, a member of the Snail family of zinc finger transcription factors, whose expression is induced by FGF, BMP, and TGF-beta. This antibody is not cross-reactive with Snail.
纯化方法
Purified
免疫原
13 amino acid peptide sequence near the centre of human Slug
SNAI2
适用: 人
ELISA, IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Western Blot: 0.5 - 2.0g/mL, detects a band of approximately 30 kDa in 293 cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, 0.02 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
储存条件
4 °C/-20 °C
储存方法
Store at 2-8 °C for up to one month or at -20 °C for longer. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Slug is a 29kD transcriptional repressor, which functions by binding to E-box motifs and inhibiting gene expression by recruiting histone deacetylase-1 (HDAC-1). Slug controls the expression of a wide variety of genes including TP53, BRCA2, PUM and CDH1, and has important roles in apoptosis and epithelial-mesenchymal transition. It is also thought to function in neural crest development. Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival.Synonyms: Neural crest transcription factor Slug, Protein snail homolog 2, SLUGH, Zinc finger protein SNAI2