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GAD 抗体 (C-Term)

Name: GAD 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN3184725
Availability: OutOfStock
Rating: 5 (1 reviews)

GAD1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody (ABIN3184725) (GAD 抗体 (C-Term))

IHC

1 image

(1 validation)

产品编号 ABIN3184725

发货至: 中国

Datasheet as PDF

抗原 See all GAD (GAD1) 抗体

GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
抗原表位
15

12

11

9

8

5

4

4

3

2

2

1

1

1

1

1

1

1

1

1
C-Term
适用
103

60

55

6

6

4

4

3

2

2

1

1

1
人, 小鼠, 大鼠
宿主
91

15

3

2
兔
Compatible Secondaries
克隆类型
89

22
多克隆
标记
55

9

4

3

3

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This GAD antibody is un-conjugated
应用范围
79

31

28

13

13

13

12

11

9

7

5

4

3

3

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

特异性

GAD67 Polyclonal Antibody detects endogenous levels of GAD67 protein.

产品特性

Rabbit Polyclonal to GAD67.

纯化方法

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

免疫原

Synthesized peptide derived from the C-terminal region of human GAD67.

亚型

IgG

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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-97) antibody
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 455-594) antibody
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibody
GAD1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal GAD1-2391 unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
GAD1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (Internal Region) antibody
Verified GAD1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibody
GAD1 适用: 人 WB, ELISA, FACS, Coat 宿主: 小鼠 Monoclonal GAD1-2563 unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 14-122) antibody
GAD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
GAD1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000,

限制

仅限研究用
生效 #103754 (Immunohistochemistry)

by

Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.

#103754

日期

2019.12.12

抗原

GAD1

Lot Number

Method validated

Immunohistochemistry

Positive Control

6 µm glass-mounted microtome sections from adult (24 months) mouse brain

Negative Control

Slices incubated overnight with the blocking solution only and then processed with the secondary antibody

Notes

Passed. The anti-GAD antibody ABIN3184725 specifically recognized GAD1 at a dilution of 1:200.

Validation Images

A and B. Detection of GAD1+ neurons in the cortex using ABIN3184725.
Full Methods
Primary Antibody

ABIN3184725

Secondary Antibody

goat anti-rabbit AF594-conjugated antibody (Thermo Fisher Scientific, A11029, lot 1942237)

Full Protocol

Perfuse mouse with 4% paraformaldehyde in 0.1 M phosphate buffer (PB) pH7.4 and post-fix spinal cord and brain samples in the same fixative for 2 h at RT.
Wash samples in PBS, dehydrate, and embed samples in paraffin wax.
Cut spinal cord and brain samples with a microtome in 6 µm-thick sections and mount sections on glass slides.
After paraffin removal, block sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) 1 h at RT.
Incubate sections with primary rabbit anti-GAD antibody (antibodies-online, ABIN3184725, lot 31965) diluted 1:200 in blocking solution ON at RT.
Wash sections 3x 5 min in 0.01 M PBS.
Incubate sections with secondary goat anti-rabbit AF594-conjugated antibody (Thermo Fisher Scientific, A11029, lot 1942237) diluted 1:200 in PBS for 1 h at RT.
Wash sections 3x 5 min in 0.01 M PBS.
Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Acquire images with Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification. Maintain Parameters for image acquisition (Exposure 2.2 s, Gain 2.3x, Saturation 1.5, Gamma 1.10) unchanged for all images.

Experimental Notes

Dilutions of 1:50, 1:100, and 1:500 were also tested but 1:200 gave the best results.
状态

Liquid

浓度

1 mg/mL

缓冲液

Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles.

储存条件

-20 °C

储存方法

Store at -20°C.
抗原

GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

别名

GAD67 (GAD1 产品)

别名

CPSQ1 antibody, GAD antibody, SCP antibody, GAD-67 antibody, cpsq1 antibody, gad1 antibody, gad1-A antibody, gad67 antibody, GAD67 antibody, GAD1 antibody, GLUTAMATE DECARBOXYLASE antibody, GLUTAMATE DECARBOXYLASE 1 antibody, MKP11.30 antibody, MKP11_30 antibody, glutamate decarboxylase antibody, EP10 antibody, GAD25 antibody, GAD44 antibody, Gad-1 antibody, Gad67 antibody, glutamate decarboxylase 1 antibody, glutamate decarboxylase 1 L homeolog antibody, glutamate decarboxylase antibody, glutamate decarboxylase 1b antibody, GAD1 antibody, gad1.1.L antibody, GAD antibody, Gad1 antibody, gad1b antibody

分子量

67 kDa

基因ID

2571

UniProt

Q99259