(1 reference)
(1 validation)-
- 抗原 See all Actin 抗体
- Actin
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适用
- 植物
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Actin antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- The antibody detects endogenous plant actin protein.
- 产品特性
- Mouse Monoclonal to Plant actin.
- 纯化方法
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 克隆位点
- Q30
- 亚型
- IgG1
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anti-Actin (AA 165-260) antibody
适用: 人, 大鼠, 小鼠, 兔, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 人, 大鼠, 小鼠, 小鸡, 犬, Cow, Pig, 豚鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin (C-Term) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: Oryza sativa, Barley, Arabidopsis, Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 367-377) antibody适用: 人, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 21-70) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 189-218) antibody适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 38-67), (N-Term) antibody适用: 人, 小鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 人, 大鼠, 小鼠, 小鸡, 兔, 犬, Cow, 猴, Pig, 豚鼠, 绵羊, Hamster, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Actin (C-Term) antibody适用: 人 IHC (p), IHC (fro) 宿主: 兔 Monoclonal S12-I unconjugated
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- 应用备注
- WB 1:2000-5000
- 限制
- 仅限研究用
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- by
- Department of Horticultural genetics and Biotechnology, Mediterranean Agronomic Institute of Chania
- No.
- #102343
- 日期
- 2017.12.19
- 抗原
- Actin
- Lot Number
- B3401
- Method validated
- Western Blotting
- Positive Control
- protein extraction from olive root tissue and tomato flower tissue
- Negative Control
- Notes
Passed. ABIN3181106 specifically recognizes the actin in both tomato flower and olive roots samples, providing a clear band at the detection with the secondary one.
- Primary Antibody
- ABIN3181106
- Secondary Antibody
- HRP conjugated rabbit anti-mouse IgG (Thermo Scientific, PA1-28902, OK1782904)
- Full Protocol
- Lyse protein extraction from 500mg olive root tissue and 80mg from tomato flower tissue in 240µl cold lysis buffer (1M Tris-Cl pH6.8, 10% SDS, 14.3M β-Merc, 0.5N EDTA, PMSF 0.5mM and 100µl proteinase inhibitor (Sigma-Aldrich, P9599-1ML)).
- Determine total protein content of the lysates using protein assay (Bradford curve).
- Denature 0.7μg/μl of total protein for 5min at 95°C 1:4 (buffer:protein) in Laemmli SDS sample buffer (Tris-Cl pH6.8, 2% SDS, 10% glycerol, 1%β-mercaptoethanol, 12.5mM EDTA, 0.02% bromophenol blue) and subsequently separate them on a freshly cast denaturing SDS-PAGE gel (30% Bis-Acrylamide, 1.5M Tris pH8.8, 10% SDS, 10% APS and TEMED) in an Bio-Rad Mini-PROTEAN 3 cell systemfor 2h at 100V, 200mA.
- Transfer proteins onto an Immobilon-P membrane (Millipore, IPVPH0010, K5CA2260G) with a Western blotting system for 1h at 100V.
- Block the membrane with 5% non-fat milk in 1x TBST for 1h at RT.
- Incubate membrane with anti-Actin antibody (antibodies-online, ABIN3181106, lot B3401) diluted 1:2500 (Flower samples) or 1:1000 (root samples) in 5% non-fat milk in 1x TBST ON at 4°C.
- Wash membrane 3x for 5min with 1x TBST.
- Incubate membrane with secondary HRP conjugated rabbit anti-mouse IgG (Thermo Scientific, PA1-28902, OK1782904) diluted 1: 5000 (flower samples) and 1:2000 (root samples) in 5% non-fat milk in 1x TBSTfor 1h at RT.
- Wash membrane 3x for 5min with 1X TBST.
- Reveal protein bands using super signal west pico (Thermo-Scientific, 34080, QF218483) on a Super RX Fuji Medical X-RAY (18cm x 24cm) film.
- Experimental Notes
The actin antibody ABIN3181106 reveals the actin protein of the expected molecular weight of antigen in lysates of tomato flower and olive root samples.
生效 #102343 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Detection of Actin in tomato flower from wt plants and different RNAi lines (A) and olive root protein extracts from different cultivars (B) using ABIN3181106 diluted 1:2500 and 1:1000 respectively.]()
Detection of Actin in tomato flower from wt plants and different RNAi lines (A) and olive root protein extracts from different cultivars (B) using ABIN3181106 diluted 1:2500 and 1:1000 respectively.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C.
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: "Dimethyl Fumarate Induces Glutathione Recycling by Upregulation of Glutathione Reductase." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6093903, (2017) (PubMed).
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: "Dimethyl Fumarate Induces Glutathione Recycling by Upregulation of Glutathione Reductase." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6093903, (2017) (PubMed).
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- 抗原
- Actin
- Abstract
- Actin 产品
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