ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
MGC75796 antibody, MGC81924 antibody, ABBP-2 antibody, ABBP2 antibody, DJ9 antibody, Dj-9 antibody, EDJ antibody, ERdj3 antibody, ERj3 antibody, ERj3p antibody, PRO1080 antibody, UNQ537 antibody, hDj-9 antibody, 1810031F23Rik antibody, AL024055 antibody, Dj9 antibody, LRRGT00084 antibody, cb954 antibody, DnaJ heat shock protein family (Hsp40) member B11 antibody, DnaJ heat shock protein family (Hsp40) member B11 S homeolog antibody, DnaJ (Hsp40) homolog, subfamily B, member 11 antibody, dnajb11 antibody, DNAJB11 antibody, dnajb11.S antibody, Dnajb11 antibody
背景
Members of the heat shock protein 40 (HSP 40) family of proteins all contain a highly conserved J domain that associates with HSP 70 and regulates the function of HSP 70 by activating its adenosine triphosphatase activity. ERdj3, an HSP 40 chaperone, is expressed in the ER lumen, where it interacts with BiP, a molecule involved in retrotranslocating proteins out of the ER. ERdj3 also associates with several other protein substrates, including unfolded light chains, a nonsecreted Ig light chain mutant and a VSV-G ts045 mutant. Shiga toxin (Stx) is a bacterial tool that enzymatically inactivates the 28S rRNA, inhibiting protein synthesis of infected cells. Stx also interacts with ERdj3 and Sec 61 to form a complex through which proteins are retrotranslocated to the cytoplasm. ERdj3 may play a role in the ER quality control system.Synonyms: APOBEC1-binding protein 2, DnaJ homolog subfamily B member 11, EDJ, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, ER-associated dnaJ protein 3, ERJ3, ERdj3, ERj3p, HEDJ, PSEC0121, PWP1-interacting protein 4, hDj9