Immunohistochemistry on frozen sections: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 500 - 1: 1000. Western blot: 1: 2000 - 1: 5000. ELISA: 1: 1000 - 1: 2000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
缓冲液
Phosphate buffered saline, BSA
储存条件
-20 °C
储存方法
Store undiluted at -20 °C only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing asthis may denature the antibody. Should this product contain a precipitate we recommendmicrocentrifugation before use. Shelf life: Six month from despatch.
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Synonyms: GFP-Tag, Green fluorescent protein